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Home / Toolbox of Mice / Fluorescent & Other Reporters / R26-CAG-LSL-Luc-tdTomato

R26-CAG-LSL-Luc-tdTomato

Background : C57BL/6Smoc

Catalog : NM-KI-18051

Strain Name :

C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-luci-2A-tdTomato)Smoc

Gene Summary

Gene Name
  • Synonyms :

    R26, ROSA26, AV258896, Gtrgeo26, Gtrosa26, Thumpd3as1

  • NCBI ID :

    14910

  • MGI ID :

    104735

  • Ensembl ID :

    ENSMUSG00000086429

  • Strain State :

    活体

Model Description

A CAG-loxP STOP-loxP-luci-2A-tdTomato-WPRE-polyA cassette was knocked into the mouse ROSA26 locus . This strain of mice can be mated with mice expressing Cre recombinase so that luciferase and tdTomato fluorescent protein could be expressed for cell tracing.

Validation Data

图1.png

Fig1. In the absence of any stimulation, the in vivo imaging system detected that R26-CAG-LSL-luci-tdTomato(+/-)；Dppa3-Cre (+/-) mice showed significant fluorescence.

图2.png

Fig2. The in vivo imaging system detected that R26-CAG-LSL-luci-tdTomato(+/-) mice showed some fluorescence. Some 'leaking' was found where luciferase were being expressed at a low level even without Cre.

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Fig. 3 Detection of tdTomato(red) in the liver, stomach, small intestine and large intestine of Dppa3Cre/+; Rosa26Luc-tdTomato/+ mice. 

image.png

Fig. 4 Detection of tdTomato(red) in various tissues of Dppa3Cre/+; Rosa26Luc-tdTomato/+ mice. Tdtomato was expressed in the brain, eye, heart, spleen, kidney, pancreas, thymus, salivary glands, lung, skin, spinal cord, testis and epididymis. (For more detailed information please contact our technical advisor.)

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