H11-CAG-LSL-mtagBFP2

Background : C57BL/6Smoc

Catalog : NM-KI-190006

Strain Name :

C57BL/6Smoc-lgs2em1(CAG-LSL-mTagBFP2)Smoc

Gene Summary

Gene Name
  • Synonyms :

    H11, Hipp11, Iis2

  • NCBI ID :

    Null

  • MGI ID :

    5461148

  • Ensembl ID :

    Null

  • Strain State :

    精子冻存

Model Description

A CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA cassette was inserted at the mouse H11 locus. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mTagBFP2-3xFLAG protein.

Validation Data

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Fig1. BFP expression in CAG-mTagBFP2/+ mouse, visualized using fluorescence imaging.

CAG-mTagBFP2/+ mouse was achieved through crossing CAG-LSL-mTagBFP2/+ mouse and Cre-expressing mouse. BFP expression in different organs of CAG-mTagBFP2/+ mouse was detected using fluorescence imaging, which suggests relatively high expression of BFP in a variety of organs. A: Images of different organs of CAG-mTagBFP2/+ mouse; B: Images of histologic sections of CAG-mTagBFP2/+ mouse under confocal microscopy.

 


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