Btdc, B7-DC, PD-L2, F730015O22Rik
胚胎冻存
Fig1.Schematic representation of gene targeting at the Pd-l2 locus.
Fig2. Genotyping of humanized PD-L2 mice by RT-PCR.
Primer P1/2
Wild type = 404 bp
Primer P3/4
Mutant type = 405 bp
M:100bp Plus DNA ladder from Transgen (BM311)
Fig 3. Splenocytes from humanized PD-L2 mice were analyzed by flow cytometry after activation.
hPD-L2+ (but not mPD-L2+) cells were detectable in the homozygous hPD-L2 mice, while hPD-L2+ and mPD-L2+ cells were both detectable in heterozygous humanized PD-L2 mice.
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