Ptf1a-CreERT2

Background : C57BL/6Smoc

Catalog : NM-KI-200118

Strain Name : C57BL/6Smoc-Ptf1aem1(CreERT2-WPRE-pA)Smoc

Gene Summary

Gene Name

Model Description

A CreERT2-WPRE-pA expression cassette was knocked into the Ptf1a gene start codon site.

Validation Data

Ptf1a-CreERT2-1.png

Fig.1 CreERT2-mediated recombination in the pancreas of Ptf1aCreERT2/+ ; Rosa26tdTomato/+ mouse. 

TdTomato(red) expression can be detected in the pancreatic acinar cells of Ptf1aCreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

Ptf1a-CreERT2-2.png

Fig.2 Detection of tdTomato (red) in various tissues of Ptf1aCreERT2/+; Rosa26tdTomato/+ mice after tamoxifen treatment. 

CreERT2-mediated recombination in the pancreatic acinar cells  can be induced by tamoxifen. Some leakiness were detected in the pancreas prior to tamoxifen exposure. Besides, tdtomato expression can be detected in a few cells of the brain, pituitary gland and stomach. Tdtomato exprssion can not be detected in thymus, heart, lung, liver, testis, kidney, intestine, brown fat, spleen, submandibular gland, and muscle. (For more detailed information please contact our technical advisor.)

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