hCB1(2)

Fig1. Detection of CB1 expression in cerebellum by RT-PCR. 

Wild type: only one band at 373 bp with primers F1/R1(mCb1); 

Homozygous: only one band at 209 bp with primers F2/R2(hCB1). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig3. Detection of human CB1 expression in CA1 in hCB1(2) knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig4. Detection of human CB1 expression in CA3 in hCB1(2) knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig5. Detection of human CB1 expression in Dentate Gyrus(DG) in hCB1(2) knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig6. Detection of human CB1 expression in Cerebellum in hCB1(2) knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.