PTP1B, PTP-1B, PTP-HA2
精子冻存
Fig.1 Detection of PTPN1 expression in spleen by RT-PCR.
Wild type: only one band at 214 bp with primers F1/R1(mPtpn1);
Homozygous: only one band at 206 bp with primers F2/R2(hPTPN1).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human PTPN1 expression in hPTPN1 knockin mice by WB.
Abbr. M, marker; WT, wild type; HO, homozygous.
Note. The anti-human PTPN1 antibody cross-reacted with mouse PTPN1 and humanized PTPN1. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.
Fig.3 Detection of human PTPN1 expression in hPTPN1 knockin mice by WB.
Abbr. M, marker; WT, wild type; HO, homozygous; PC, positive control, Hela cells.
Note. The anti-human PTPN1 antibody cross-reacted with mouse PTPN1 and humanized PTPN1. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.
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