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Fig.1 Detection of MC4R expression in brain by RT-PCR.
Wild type: only one band at 264 bp with primers F1/R1(mMc4r);
Homozygous: only one band at 342 bp with primers F2/R2(hMC4R).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human MC4R expression in brain in hMC4R mice by WB.
Abbr. M, marker; WT, wild type; HO, Homozygous.
Note. The anti-human MC4R antibody cross-reacted with mouse MC4R and humanized MC4R.
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