hIL2/hIL2RA/hIL2RB/hIL2RG

Nomenclature

C57BL/6Smoc-Il2tm3(hIL2)Il2ratm1(hIL2RA)Il2rgem1(IL2RG)Il2rbtm1(hIL2RB)Smoc

Cat. NO.

NM-HU-210415

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
IL2

Synonyms

Il-2

NCBI ID

16183

MGI ID

96548

Ensembl ID

ENSMUSG00000027720 

Pubmed

IL2

Human Ortholog

IL2

Gene Symbol
IL2RA

Synonyms

CD25; Il2r; IL-2R alpha chain; Ly-43

NCBI ID

16184

MGI ID

96549

Ensembl ID

ENSMUSG00000026770

Pubmed

IL2RA

Human Ortholog

IL2RA

Gene Symbol
IL2RB

Synonyms

p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta

NCBI ID

16185

MGI ID

96550

Ensembl ID

ENSMUSG00000068227

Pubmed

IL2RB

Human Ortholog

IL2RB

Gene Symbol
IL2RG

Synonyms

gc; p64; [g]c; CD132; gamma(c)

NCBI ID

16186

MGI ID

96551

Ensembl ID

ENSMUSG00000031304

Pubmed

IL2RG

Human Ortholog

IL2RG

Gene Symbol
Il2ra

Synonyms

CD25; Il2r; IL-2R alpha chain; Ly-43

NCBI ID

16184

MGI ID

96549

Ensembl ID

ENSMUSG00000026770

Pubmed

Il2ra

Human Ortholog

IL2RA

Model Description

hIL2/hIL2RA(NM-HU-200275) was crossed with hIL2RA/hIL2RB/hIL2RG(NM-HU-210414) to generate hIL2/hIL2RA/hIL2RB/hIL2RG mice.

Validation Data

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Fig1. Detection of hIL-2 expression by ELISA in the hIL-2 knockin mice (Wild type and heterozygous hIL-2 KI mice were administrated with concanavalin).

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Fig2. Intracellular staining of IL-2 by FACS post stimulation with anti-mCD3/anti-mCD28 (In collaboration with CrownBio).

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Fig3. IL-2RA humanized mouse expresses hlL-2RA (hCD25) on naïve Treg in blood (In collaboration with CrownBio).

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Fig4. hlL-2RA is highly expressed on activated Tregs in spleen in the IL-2RA huamnzied mouse (In collaboration with CrownBio).

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Fig5. hlL-2RA is highly expressed on activated NK cells in spleen in the IL-2RA humanized mouse (In collaboration with CrownBio).

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Fig6. Immunotype in hIL2/hIL2RA/hIL2RB/hIL2RG qKI mice.

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Fig7. Immunotype in hIL2/hIL2RA/hIL2RB/hIL2RG qKI mice in Blood.

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Fig8. Immunotype in hIL2/hIL2RA/hIL2RB/hIL2RG qKI mice in Spleen.

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Fig9. Detection of Signaling integrity in hIL2/hIL2RA/hIL2RB/hIL2RG qKI mice.

Note. Splenocytes of C57BL/6 mice were treated with anti-mCD3 and mIL-2(10 ng/ml) for 72h. Splenocytes of hIL2/hIL2RA/hIL2RB/hIL2RG qKI mice were treated with anti-mCD3 and hIL-2(10 ng/ml) for 72h.

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