Fig.1 hTROP2 expression on in vitro cultured cells of CT26-hTROP2.
Fig.2 In vivo validation of CT26-hTROP2 in BALB/c mice.BALB/c mice were inoculated with CT26-hTROP2 subcutaneously at 3×106 cells/mouse inoculum for tumor development. The day of vaccination for mice is defined as Day 0, and tumor measurement and weight measurement begin on the 7th day after vaccination, twice a week.
Fig.3 hTROP2 expression in tumors after in vivo growth of CT26-hTROP2 in wild type BALB/c mice.When the MTV reaches 500-800 mm3, take one tube from each group and send it to FACS for testing (Markers: L/D, mCD45, hTROP2). A，the MTV reaches 800 mm3. B，the MTV reaches 1500 mm3.
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