Figure 1. Expression of human PD-L1 on EMT6-hPD-L1 cells was confirmed by flow cytometry.
EMT6-hPD-L1 cells and wild type EMT6 cells were stained with species-specific anti-PD-L1 antibodies. FACS analysis shows that human PD-L1 but not mouse PD-L1 was exclusively detectable on EMT6-hPD-L1 cells.
Figure 2. In vivo tumor growth curves in humanized EMT6-hPD-L1 syngeneic model.
BALB/c mice were subcutaneously injected 5×105 EMT6-hPD-L1 cells compared with wild type EMT6 cells as control. Tumor growth was monitored by measuring tumor size from day 7 after subcutaneous implantation.
Data shows that there were no significant differences between EMT6-hPD-L1 cells and wild type EMT6 cells in either tumorigenicity or tumor growth.
Figure 3. FACS analysis of PD-L1 expression on tumor cells derived from humanized EMT6-hPD-L1 syngeneic model with species-specific anti-PD-L1 antibodies.
Data shows that neither human PD-L1 nor mouse PD-L1 was detectable in EMT6-hPD-L1 knock-in tumor. This EMT6-hPD-L1 cell line can only be used for in vitro validation not for in vivo experiments.
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