Strain Model Specification

Gpr65-IRES-Cre

Strain Name C57BL/6Smoc-Gpr65em1(IRES-iCre)Smoc
Catalog Number NM-KI-200135
Genetic Background C57BL/6Smoc
Gene Symbol Gpr65
Synonyms Dig1, TDAG8, Gpcr25
NCBI ID 14744 ↗
MGI ID 108031 ↗
Ensembl ID ENSMUSG00000021886 ↗
Strain State Sperm cryopreservation
Research Application Cre recombinase tool; The G-protein-coupled receptor(GPR65) neurons  innervate intestinal villi and gastric mucosa to sense nutrients. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre.

Validation Data

image.png

Fig. 1 Cre-mediated recombination in the intestine of Gpr65Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the large intestine and small intestine derived from Gpr65Cre/+; Rosa26tdTomato/+ mouse.

image.png

Fig. 2 Detection of tdTomato(red) in various tissues of Gpr65Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the pituitary gland, heart, liver, lung, stomach, salivary gland, kidney, pancreas, large intestine, skin, testis and epididymis. TdTomato can also be detected in individual cells of the thymus and spleen. Tdtomato expression can not be observed in brown fat. (For more detailed information please contact our technical advisor.)