hADORA2A

Fig1. Detection of ADORA2A expression in thymus and spleen by RT-PCR. Wild type: only one band at 184 bp with primers F1/R1 (mAdora2a); Homozygous: only one band at 234 bp with primers F2/R2 (hADORA2A); Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Detection of hADORA2A expression in Myeloid cells and activated T cells in hADORA2A KI mice. Note. Splenocytes from C57BL/6 and homozygous hADORA2A mice were stimulated with anti-mCD3 antibody in vitro. The anti-human ADORA2A antibody cross-reacted with mADORA2A and humanized ADORA2A.

Fig3. Detection of hADORA2A expression in activated B cells and NK cells in hADORA2A KI mice. Note. Splenocytes from C57BL/6 and homozygous hADORA2A mice were stimulated with anti-mCD3 antibody in vitro. The anti-human ADORA2A antibody cross-reacted with mADORA2A and humanized ADORA2A.

Fig4. Detection of mA2AR expression in hippocampus(CA3) in C57BL/6 mice by IHC. Abbr. HO/HO, homozygous; +/+, wild type. Note. The anti-mouse ADORA2A Antibody cross-reacted with mADORA2A and humanized ADORA2A.

Fig5. Detection of hA2AR expression in hippocampus(CA3) in HO hADORA2A KI mice by IHC. Abbr. HO/HO, homozygous; +/+, wild type. Note. The anti-human ADORA2A Antibody cross-reacted with mADORA2A and humanized ADORA2A.