C5, He, C5a, Hfib2
活体
Fig 1. Detection of C5 expression by RT-PCR.
Wild type: only one band at 167 bp with primers F2/R2(mC5);
Homozygous: only one band at 337 bp with primers F1/R1(hC5).
Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Fig 2. Analysis of humanized C5 and humanized C5a expression in serum in the humanized C5 mouse by ELISA.
The homozygous hC5 KI mice were treated with LPS or NS (3mg/kg, i.p.) for 6 hours.
Fig 3. Detection of hC5 expression in eyes in hC5 KI mice by IF (n=3/group).
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