B, Bf, C2, Fb, H2-Bf, AI195813, AI255840
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Fig1. Detection of CFB expression in liver by RT-PCR.
Wild type: only one band at 441 bp with primers F1/R1(mCfb);
Homozygous: only one band at 442 bp with primers F2/R2(hCFB);
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of human and mouse CFB expression in serum by ELISA (n=2).
Abbr. HO, homozygous; WT, wild type.
Note: The mouse CFB ELISA kit cross-reacted with mCFB and humanized CFB.
Fig3. Detection of hCFB expression in serum by ELISA(n=3/group).
Abbr. HO, homozygous; WT, wild type
Fig4. Detection of liver CFB mRNA remaining at Day 7 (A) and serum protein level (B) after single dose of test drug (n=3/group). (Data from a collaborator)
Fig5.Partial representative pictures of liver and kidney pathology in hCFB knockin mice. No obvious lesions were observed in hCFB knockin mice.
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