Strain Model Specification

hIL12A

Strain Name C57BL/6Smoc-Il12atm3(hIL12A)/Smoc
Catalog Number NM-HU-2000056
Genetic Background C57BL/6Smoc
Gene Symbol Il12a
Synonyms p35, Ll12a, Il-12a, IL-12p35
NCBI ID 16159 ↗
MGI ID 96539 ↗
Ensembl ID ENSMUSG00000027776 ↗
Strain State Embryo cryopreservation
Research Application Cytokine humanization model；drug screening

Validation Data

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Fig1. Detection of IL12A expression in spleen by RT-PCR. 

Wild type: only one band at 198 bp with primers F1/R1(mIl12a);

Homozygous: only one band at 488 bp with primers F2/R2(hIL12A); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of human IL12P70 and mouse IL12P70 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hIL12A/hIL12B and C57BL/6 mice were i.p. injected with LPS for 2h.