hIL21

Background : C57BL/6Smoc

Catalog : NM-HU-200255

Strain Name :

C57BL/6Smoc-Il21tm1(hIL21)Smoc

Gene Summary

Gene Name

Model Description

The endogenous mice Il21 gene was replaced by human IL21 gene.

Validation Data

image.png

Fig1. Detection of IL21 expression in splenocyte  by RT-PCR. 

Wild type: only one band at 272 bp with primers F1/R1(mIl21);

Homozygous: only one band at 304 bp with primers F2/R2(hIL21); 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig2. Detection of hIL21 expression in splenocyte culture supernatants by ELISA (n=3). 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

Note. hIL21 and C57BL/6 mice were stimulated with ConA in vitro.

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