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Fig.1 Detection of INHBE expression in liver by RT-PCR.
Wild type: only one band at 192 bp with primers F1/R1 (mInhbe);
Homozygous: only one band at 217 bp with primers F2/R2 (hINHBE).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human INHBE expression in liver in hINHBE knockin mice by WB.
Abbr. M, marker; WT, wild type; HO, homozygous; PC, positive control, Hep G2 cells.
Note. The anti-human INHBE Antibody cross-reacted with mouse INHBE and humanized INHBE. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.
Fig.3 Detection of hINHBE expression in serum by ELISA (n=3).
Note. The human INHBE Elisa kit cross-reacted with mouse INHBE and humanized INHBE.
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