hPD-1/hCTLA-4

  PD-1 expression in stimulated T cells  

Fig1. The expression of human PD-1 and CTLA-4 in double humanized PD-1&CTLA-4 mice was confirmed by FACS.

Efficacy studies

Fig2. In vivo validation of double humanized PD-1&CTLA-4 mice. Double humanized mice were inoculated with MC38 cells, and randomly assigned to different groups (n=8) when the tumor grew to a volume of 100 mm3. A combinatorial treatment of anti-CTLA-4 and anti-PD-1 demonstrated a noticeable efficacy improvement compared to the same dose of single agent (top) without affecting the animal body weight (Bottom).

Re-challenge studies

Figure 3. As shown in the previous figure, animals of group 1-4 received different doses of either single agent or combinatorial treatment. This graph showed the progression of the tumor implanted in the cured, G4 mice, implicating a systematic anti-tumor effect induced by the combinatorial antibody treatment.