Strain Model Specification

hPD-L1/hCTLA-4

Strain Name C57BL/6Smoc-Ctla4tm1(hCTLA4)Cd274tm1(hPD-L1)Smoc
Catalog Number NM-HU-00102
Genetic Background C57BL/6Smoc
Synonyms Cd152, Ly-56, Ctla-4|B7h1, Pdl1, Pdcd1l1, Pdcd1lg1, A530045L16Rik,
NCBI ID 12477,60533, ↗
MGI ID 88556,1926446, ↗
Ensembl ID ENSMUSG00000026011,ENSMUSG00000016496, ↗
Strain State Embryo cryopreservation
Research Application Immunotherapy,cancer research,drug screening

Validation Data

PD-L1&CTLA4-HU.png


Fig1 Splenocytes from homozygous hPDL1/hCTLA4 mice were analyzed by flow cytometry after activation. mPDL1+ and mCTLA4+ were detectable in wild type (WT) C57BL/6, while hPDL1+ and hCTLA4+ cells were detectable in the homozygous hCTLA4/h4-1BB mice. 

ctla4-pdl1.png

ctla4-pdl1-2.png

Fig2  hCTLA-4/PD-L1 mice+ MC38 cells efficacy studies. We have developed a CTLA-4/PD-L1 dKI humanized model and tested the combined treatment of Yervoy and Tecentriq, and then observed improved efficacy in the combination treatment.