Figure 1. Expression of human CD19 on MC38-hCD19 cells was confirmed by flow cytometry.
MC38-hCD19 cells and wild type MC38 cells were stained with species-specific anti-CD19 antibody. FACS analysis shows that human CD19 expression on MC38-hCD19 cells was approximately 90%.
Figure 2. In vivo tumor growth curves in humanized MC38-hCD19 syngeneic model.
C57BL/6 mice were subcutaneously injected 1×106 MC38-hCD19 cells compared with wild type MC38 cells as control. Tumor growth was monitored by measuring tumor size from day 6 after subcutaneous implantation.
Data shows that there were no significant differences between MC38-hCD19 cells and wild type MC38 cells in either tumorigenicity or tumor growth.
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