Strain Model Specification

Nkx2-5-IRES-Cre

Strain Name C57BL/6Smoc-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc
Catalog Number NM-KI-200152
Genetic Background C57BL/6Smoc
Gene Symbol Nkx2-5
Synonyms Csx, Nkx2.5, tinman, Nkx-2.5
NCBI ID 18091 ↗
MGI ID 97350 ↗
Ensembl ID ENSMUSG00000015579 ↗
Strain State Sperm cryopreservation
Research Application Cre recombinase tool; Nkx2.5 acts as a key transcription factor and plays essential roles for heart formation. By mating the reporter mice with Cre-expressing mice, Nkx2-5 positive cells derived from double-positive mice were permanently labeled by fluorescent protein. When crossed with mice carrying a targeted floxed alleles, this strain is useful in eliminating tissue-specific conditional expression of the gene.

Validation Data

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Fig. 1 Cre-mediated recombination in the heart of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the myocardial cells and vascular cells of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 2 Detection of tdTomato(red) in various tissues of Nkx2-5Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the heart, bronchus, pulmonary alveoli and submandibular gland. Tdtomato expression can be also detected in individual cells derived from the brain, retina, skin, kidney, pancreas, intestine, colon, spleen and thymus, except for pituitary and muscle. (For more detailed information please contact our technical advisor.)