R26-CAG-LSL-RSR-tdTomato-2A-DTR

Fig1. Immunostaining for tdTomato on sections of multiple tissues or organs collected from ACTB-Cre;R26-LR-tdT-DTR mice (upper panel) or CAG-Dre;R26-LR-tdT-DTR mice (lower panel). 

Fig2. DTR-mediated cardiomyocyte ablation after DT administration. 

A, a schematic figure showing experimental design. B, whole-mount view of triple transgenic mice treated with PBS or DT. Yellow scale bar represents 2000 μm.  C, immunostaining for DTR and tdTomato on heart sections of triple transgenic mice. White scale bar represents100 μm.  D, immunostaining for cTnT and tdTomato on heart sections of triple transgenic mice. White scale bar represents 100 μm. E, immunostaining for Tunel and tdTomato on heart sections. White scale bar represents 100 μm.

Fig3. DTR-mediated specific ablation of Cyp2e1⁺ peri-central hepatocytes.

A, a cartoon figure showing the mouse’s mating strategy. B, whole-mount epifluorescence view of livers collected from mice treated with DT or PBS. Yellow scale bar represents 2000 μm.  C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections treated with PBS. White scale bar represents 100 μm. C, central vein. E, immunostaining for CYP2E1, tdTomato, and E-cad on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm. F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison of tdTomato+ hepatocytes (PBS group). G, immunostaining for Tunel and tdTomato on liver sections from mice treated with PBS or DT. White scale barrepresents 100 μm.

Fig4. DTR-mediated specific ablation of Mfsd2a⁺ peri-portal hepatocytes.

A, a schematic figure showing the genetic recombination of the R26-LR-tdT-DTR allele by Mfsd2a-CreER and AAV8-TBG-Dre.  B, whole-mount fluorescent view of livers collected from mice treated with PBS or DT. Yellow scale bar represents 2000 μm.  C and D, immunostaining for tdTomato, HNF4a (E), or DTR (F) on liver sections of mice treated with PBS.White scale bar represents100 μm. E, immunostainingfor E-cad and tdTomato on liver sections from mice treated with PBS or DT. White scale bar represents 100 μm.  F, quantification of the percentage of tdTomato+ hepatocytes (DT group) in comparison with tdTomato+ hepatocytes (PBS group). n = 5.  G, immunostaining for Tunnel and tdTomato on liver sections of mice treated with PBS or DT. White scale bar represents 100 μm.

*The above data is derived from: Wang H, He L, Li Y, et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo. J Biol Chem. 2022;298(6):101965. doi:10.1016/j.jbc.2022.101965