Strain Model Specification

Rag1-KO(Rag1-EGFP)

Strain Name B6;129S-Rag1tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc
Catalog Number NM-KI-00069
Genetic Background B6;129S
Gene Symbol Rag1
Synonyms Rag-1
NCBI ID 19373 ↗
MGI ID 97848 ↗
Ensembl ID ENSMUSG00000061311 ↗
Strain State Repository Live
Research Application Immunodeficiency,tumor-bearing model

Validation Data

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Fig.1 Construction strategy of Rag1-KO.

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Fig.2 Loss of T and B cells in peripheral blood of Rag1-KO and Rag2-KO mice.

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Fig.3 Loss of T and B cells in Spleen of Rag1-KO and Rag2-KO mice.

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Fig.4 Loss of T and B cells in thymus of Rag1-KO and Rag2-KO mice.

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Fig.5 Detection of EGFP expression in CD45+ cells in spleen, blood, thymus, and bone marrow of 8.7-week-old female WT and HO Rag1-KO(Rag1-EGFP) mice.

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Fig.6 Detection of mouse IgG(A) and IgM(B) expression in serum by ELISA. (male, 5~6wks, n=3)

Abbr. HO, homozygous; WT, wild type; N.D. not detected.

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Fig.7 Tumorigenicity results following inoculation with A549 tumor cells in Balb/c nude, NOD-Scid, Rag1-KO and Rag2-KO mice.


Table1. Complete blood count of Rag1-KO (Rag1-EGFP) mice

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Table 2. Serum biochemical analysis of Rag1-KO (Rag1-EGFP) mice.

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