Strain Model Specification

Rag2-KO(Rag2-EGFP)

Strain Name B6;129S-Rag2tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc
Catalog Number NM-KI-00070
Genetic Background B6;129S
Gene Symbol Rag2
Synonyms Rag-2
NCBI ID 19374 ↗
MGI ID 97849 ↗
Ensembl ID ENSMUSG00000032864 ↗
Strain State Embryo cryopreservation
Research Application Immunodeficiency,tumor-bearing model

Validation Data

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Figure 1. Generation strategy of Rag2 gene knockout mice.

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Figure 2  Splenocytes cells of C57BL/6J, NOD-SCID, Rag1-/-, and Rag2-/-  mice were isolated. Fractions of T and B cells were characterized using flow cytometry. 


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Figure3. Complete deletion of T and B cells in the blood of Rag1-KO/ Rag2-KO mice.

(A) The peripheral blood samples of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.

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Figure4. Complete deletion of T and B cells in the spleen of Rag1-KO/ Rag2-KO mice.

(A) The splenocytes of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.

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Figure5. Complete deletion of T and B cells in the thymus gland of Rag1-KO/ Rag2-KO mice.

(A) The thymocyte of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.

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Figure6. EGFP expression of Rag2 knockout mice was detected.

The results showed that there were EGFP-positive cells in the thymus and spleen of Rag2 KO mice, but the EGFP-positive cells were both CD4-negative and CD8-negative cells.

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Figure7.The establishment of tumor models using A549 lung cancer cells is more effective in Rag1-/- or Rag2-/-   mice.