Trp53-R172H

Pancreatic ductal adenocarcinoma

The KPC mouse is an established and clinically relevant model of pancreatic ductal adenocarcinoma (PDAC) which develops many key features observed in human PDAC including pancreatic intraepithelial neoplasia alongside a robust inflammatory reaction including exclusion of effector T cells. Metastases are observed in around 80% of KPC animals located primarily in the liver and lungs. Mutations in both KRAS and TP53 genes are found in around 80% and 70% of all human PDACs respectively. 

Trp53-(R172H) (NM-KI-18028)、Kras-(LSL-G12D) (NM-KI-190003)were crossed with Pdx1-(Avi-CreERT2) (NM-KI-18042) to generate KPC mice.

Fig 1 The spontaneous pancreatic tumor of KPC mouse model with large volume, uneven surface and multiple nodular projections.

Fig 2 HE staining of pancreatic tumor from KPC mouse model.

The tumor cells from KPC mouse model demonstrated disorderly arranged pancreatic cells, irregular tissue structure, dilated pancreatic ducts, inflammatory cells infiltration and stromal fibrosis as was seen in pancreas adenocarcinoma.

Figure 3 Changes in pancreatic tumor volume of tumor-burdened mice.

Wild-type mice were inoculated with pancreatic tumor cells from KPC mice, and pancreatic tumors grew in size over time.