Rag1-KO(Rag1-EGFP)
Nomenclature
B6;129S-Rag1tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc
Cat. NO.
NM-KI-00069
Strain State
Repository Live
Gene Summary
Gene Symbol
Rag1
Model Description
Validation Data
Figure 1. Generation strategy of Rag1 gene knockout mice.
Figure 2 Splenocytes cells of C57BL/6J, NOD-SCID, Rag1-/-, and Rag2-/- mice were isolated. Fractions of T and B cells were characterized using flow cytometry.
Figure3. Complete deletion of T and B cells in the blood of Rag1-KO/ Rag2-KO mice.
(A) The peripheral blood samples of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.
Figure4. Complete deletion of T and B cells in the spleen of Rag1-KO/ Rag2-KO mice.
(A) The splenocytes of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.
Figure5. Complete deletion of T and B cells in the thymus gland of Rag1-KO/ Rag2-KO mice.
(A) The thymocyte of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.
Figure6.The establishment of tumor models using A549 lung cancer cells is more effective in Rag1-/- or Rag2-/- mice.
Table 1. Blood routine tests in Rag1-KO(Rag1-EGFP).
Table2. Blood biochemistry in Rag1-KO(Rag1-EGFP).
Publications
Programmed death protein 1 is essential for maintaining the anti-inflammatory function of infiltrating regulatory T cells in a murine spinal cord injury model
References:Journal of Neuroimmunology
Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus
References:Nature Communications
Interleukin-17 Regulates Neuron-Glial Communications, Synaptic Transmission, and Neuropathic Pain after Chemotherapy
References:Cell Reports
Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells
References:Frontiers in Immunology
Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling
References:Frontiers in Immunology
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