hIL12A

Nomenclature

C57BL/6Smoc-Il12atm3(hIL12A)/Smoc

Cat. NO.

NM-HU-2000056

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Il12a

Model Description

The endogenous mouse Il12a gene was replaced by human IL12A gene.
Research Application:Cytokine humanization model;drug screening

Validation Data

image.png

Fig1. Detection of IL12A expression in spleen by RT-PCR. 

Wild type: only one band at 198 bp with primers F1/R1(mIl12a);

Homozygous: only one band at 488 bp with primers F2/R2(hIL12A); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig2. Detection of human IL12P70 and mouse IL12P70 expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hIL12A/hIL12B and C57BL/6 mice were i.p. injected with LPS for 2h.


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