hIL33

Nomenclature

C57BL/6Smoc-Il33tm2(hIL33)/Smoc

Cat. NO.

NM-HU-2000069

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Il33

Model Description

The endogenous mouse Il33 gene was replaced by human IL33 gene.
Research Application:Cytokine humanization model;drug screening

Validation Data

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Fig1. Detection of IL33 expression in lung by RT-PCR. Wild type: only one band at 287 bp with primers F1/R1(mIl33);Homozygous: only one band at 311 bp with primers F2/R2(hIL33); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig 2. Detection of hIL33 expression in lung homogenate by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type.Note. hIL33 and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 4h.

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Fig 3. Detection of mIL33 expression in lung homogenate by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL33 and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 4h.


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