H11-CAG-LSL-Myc

Nomenclature

C57BL/6Smoc-Igs2em1(CAG-LSL-Myc)Smoc

Cat. NO.

NM-KI-00039

Strain State

Repository Live

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Gene Summary

Gene Symbol

Synonyms

H11，Hipp11，Iis2

NCBI ID

Null

MGI ID

5461148

Ensembl ID

Null

Pubmed

Model Description

The c-Myc gene (also known as Myc) is abnormally expressed in many tumors and plays a critical role in the regulation of cell proliferation, growth and metabolism, gene instability, stimulation of angiogenesis, malignant transformation, cell differentiation and apoptosis. These mice harbor a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven Myc oncogene. The targeted mutation was inserted into the Hipp11(H11) locus by homologous recombination. Myc is expressed when bred to mice that express Cre recombinase.
Research Application：tumor-bearing model,cancer research

Validation Data

Using the Crispr technology, the conditional overexpression structure of CAG promoter-loxp-STOP-loxp-Myc-polyA was inserted into the H11 locus to generate an H11-LSL-Myc mouse model. Located on mouse chromosome 11, the H11 locus is similar to Rosa26 and can be used to express a wide range of exogenous genes. The Myc gene can be highly expressed in Cre-expressing tissues after mating the H11-LSL-Myc mice with Cre mice. 

For example: After mating H11-LSL-Myc mice with Alb-cre mice, a liver cancer can be spontaneously developed at 2 months old. This mouse model can be used in the establishment of tumor models and tumor research.

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Fig1. Schematic diagram of H11-LSL-Myc knockin cassette. 

myc-luci-alb.png

Fig2  Representative in vivo bioluminescence imaging showing the growth of tumors in C57BL/6 mice inoculated with hepatocellular carcinoma tissue from H11-LSL-Myc; Rosa26-LSL-Luc-EGFP; Alb-Cre mice.

Publications

A method to establish a c-Myc transgenic mouse model of hepatocellular carcinoma
References：MethodsX

Loss of Mettl3 enhances liver tumorigenesis by inducing hepatocyte dedifferentiation and hyperproliferation
References：Cell Reports

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