R26-CAG-2xKRAB-dCas9-IRES-EGFP

Nomenclature

C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-Loxp-2xKRAB-dCas9-IRES-EGFP)Smoc

Cat. NO.

NM-KI-18007

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol
Gt(ROSA)26Sor

Synonyms

R26，ROSA26，AV258896，Gtrgeo26，Gtrosa26，Thumpd3as1

NCBI ID

14910

MGI ID

104735

Ensembl ID

ENSMUSG00000086429

Pubmed

Gt(ROSA)26Sor

Model Description

These mice harbor a CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted.
Research Application：CRISPRi,gene regulation tool

Validation Data

图片1.png

Validation of 2xKRAB-dCas9 expression by  Western blot

Rosa26- CAG-LSL-2xKRAB-dCas9 mice were crossed with Dppa3-Cre mice to delete STOP cassette.

Anti-Cas9 (1).jpg

Validation of EGFP expression by fluorescence assay

CRISPRi—inhibitor： dCas9-KRAB_06.png

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