R26-CAG-LSL-Luc-tdTomato

Nomenclature

C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-luci-2A-tdTomato)Smoc

Cat. NO.

NM-KI-18051

Strain State

Repository Live

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Gene Summary

Gene Symbol
Gt(ROSA)26Sor

Synonyms

R26，ROSA26，AV258896，Gtrgeo26，Gtrosa26，Thumpd3as1

NCBI ID

14910

MGI ID

104735

Ensembl ID

ENSMUSG00000086429

Pubmed

Gt(ROSA)26Sor

Model Description

A CAG-loxP STOP-loxP-luci-2A-tdTomato-WPRE-polyA cassette was knocked into the mouse ROSA26 locus . This strain of mice can be mated with mice expressing Cre recombinase so that luciferase and tdTomato fluorescent protein could be expressed for cell tracing.
Research Application：Cell tracing

Validation Data

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Fig1. In the absence of any stimulation, the in vivo imaging system detected that R26-CAG-LSL-luci-tdTomato(+/-)；Dppa3-Cre (+/-) mice showed significant fluorescence.

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Fig2. The in vivo imaging system detected that R26-CAG-LSL-luci-tdTomato(+/-) mice showed some fluorescence. Some 'leaking' was found where luciferase were being expressed at a low level even without Cre.

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Fig. 3 Detection of tdTomato(red) in the liver, stomach, small intestine and large intestine of Dppa3Cre/+; Rosa26Luc-tdTomato/+ mice. 

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Fig. 4 Detection of tdTomato(red) in various tissues of Dppa3Cre/+; Rosa26Luc-tdTomato/+ mice. Tdtomato was expressed in the brain, eye, heart, spleen, kidney, pancreas, thymus, salivary glands, lung, skin, spinal cord, testis and epididymis. (For more detailed information please contact our technical advisor.)

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