H11-CAG-LSL-mtagBFP2

Nomenclature

C57BL/6Smoc-lgs2em1(CAG-LSL-mTagBFP2)Smoc

Cat. NO.

NM-KI-190006

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol

Synonyms

H11，Hipp11，Iis2

NCBI ID

Null

MGI ID

5461148

Ensembl ID

Null

Pubmed

Model Description

A CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA cassette was inserted at the mouse H11 locus. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mTagBFP2-3xFLAG protein.
Research Application：Fluorescent reporter Mice

Validation Data

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Fig1. BFP expression in CAG-mTagBFP2/+ mouse, visualized using fluorescence imaging.

CAG-mTagBFP2/+ mouse was achieved through crossing CAG-LSL-mTagBFP2/+ mouse and Cre-expressing mouse. BFP expression in different organs of CAG-mTagBFP2/+ mouse was detected using fluorescence imaging, which suggests relatively high expression of BFP in a variety of organs. A: Images of different organs of CAG-mTagBFP2/+ mouse; B: Images of histologic sections of CAG-mTagBFP2/+ mouse under confocal microscopy.

 


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