Nos1-CreERT2

Nomenclature

C57BL/6Smoc-Nos1em1(CreERT2-SV40polyA)Smoc

Cat. NO.

NM-KI-200091

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol
Nos1

Model Description

A CreERT2-SV40polyA expression cassette was knocked into the Nos1 gene start codon site.
Research Application:Nos1, the neurogenic nitric oxide synthase, is a key enzyme responsible for the production of the signaling molecule nitric oxide (NO) by neurons. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre after tamoxifen treatment.

Validation Data

NM-KI-200091;Nos1-CreERT2-1.png

Fig. 1 CreERT2-mediated recombination in the brian of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the NOS1 positive neurons of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200091;Nos1-CreERT2-2.png

Fig. 2 CreERT2-mediated recombination in the large intestine of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer of the wall of the large intestine derived from Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200091;Nos1-CreERT2-3.png

Fig. 3 CreERT2-mediated recombination in the small intestine of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer of the wall of the small intestine derived from Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

NM-KI-200091;Nos1-CreERT2-4.png

Fig. 4 Detection of tdTomato(red) in various tissues of Nos1CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in some cells of the brain and intestine. Some leakiness were detected prior to tamoxifen exposure. Besides, tamoxifen-dependent cre recombinase expression can also be detected in some cells derived from eyes, lung, submandibular gland, kidney, spleen, thymus, muscle and ovary, except for heart, brown fat tissue and liver. (For more detailed information please contact our technical advisor.)


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