Nkx2-5-IRES-Cre

Nomenclature

C57BL/6Smoc-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc

Cat. NO.

NM-KI-200152

Strain State

Sperm cryopreservation

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Gene Summary

Gene Symbol
Nkx2-5

Synonyms

Csx; Nkx2.5; tinman; Nkx-2.5

NCBI ID

18091

MGI ID

97350

Ensembl ID

ENSMUSG00000015579

Pubmed

Nkx2-5

Human Ortholog

NKX2-5

Model Description

A IRES-Cre-WPRE-polyA expression cassette was knocked into the Nkx2-5 gene stop codon site.
Research Application：Cre recombinase tool; Nkx2.5 acts as a key transcription factor and plays essential roles for heart formation. By mating the reporter mice with Cre-expressing mice, Nkx2-5 positive cells derived from double-positive mice were permanently labeled by fluorescent protein. When crossed with mice carrying a targeted floxed alleles, this strain is useful in eliminating tissue-specific conditional expression of the gene.

Validation Data

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Fig. 1 Cre-mediated recombination in the heart of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the myocardial cells and vascular cells of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 2 Detection of tdTomato(red) in various tissues of Nkx2-5Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the heart, bronchus, pulmonary alveoli and submandibular gland. Tdtomato expression can be also detected in individual cells derived from the brain, retina, skin, kidney, pancreas, intestine, colon, spleen and thymus, except for pituitary and muscle. (For more detailed information please contact our technical advisor.)


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