Strain Model Specification

hIL2

Strain Name C57BL/6Smoc-Il2tm3(hIL2)Smoc
Catalog Number NM-HU-190048
Genetic Background C57BL/6Smoc
Gene Symbol Il2
Synonyms IL-2
NCBI ID 16183 ↗
MGI ID 96548 ↗
Ensembl ID ENSMUSG00000027720 ↗
Strain State Embryo cryopreservation
Research Application Immunotherapy,cancer research,drug screening

Validation Data

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Fig1. Detection of IL2 expression by RT-PCR. 

Wild type: only one band at 255 bp with primers F2/R2(mIl2); Homozygous: only one band at 320 bp with primers F1/R1(hIL2). 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Expression of human IL2 in the Serum of IL2-HU heterozygous mice is detected by ELISA.

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Fig3. Expression of murine IL2 in the Serum of IL2-HU heterozygous mice and  is detected by ELISA.

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Fig4. Splenocytes from humanized IL2 mice were analyzed by flow cytometry after activation. 

hIL2+ (but not mIL2+) cells were detectable in the homozygous hPD-L2 mice.