Strain Model Specification

hMASP2

Strain Name C57BL/6Smoc-Masp2tm1(hMASP2)/Smoc
Catalog Number NM-HU-2000076
Genetic Background C57BL/6Smoc
Gene Symbol Masp2
Synonyms MAp19, MASP-2
NCBI ID 17175 ↗
MGI ID 1330832 ↗
Ensembl ID ENSMUSG00000028979 ↗
Strain State Embryo cryopreservation
Research Application Immunotherapy,drug screening

Validation Data

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Fig1. Detection of MASP2 expression in liver by RT-PCR. 

Wild type: only one band at 479 bp with primers F1/R1(mMasp2);

Homozygous: only one band at 338 bp with primers F2/R2(hMASP2); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

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Fig2. Detection of human MASP2 expression in Blood (A) and Liver (B) of WT C57BL/6 and HO hMASP2 mice by Western blot. (7 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig3. Detection of hMASP2 expression in liver in HO hMASP2 mice by IHC. (female, 13 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig4. Detection of hMASP2 expression in kidney in HO hMASP2 mice by IHC. (female, 13 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig5. Detection of IgA expression in liver in HO hMASP2 mice by IHC. (female, 13 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig6. Detection of IgA expression in kidney in HO hMASP2 mice by IHC. (female, 13 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig7. No obvious pathological features were found in liver in HO hMASP2 mice. (female, 13 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig8. No obvious pathological features were found in kidney in HO hMASP2 mice. (female, 13 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig9. Detection of mC3 expression in serum by ELISA. (female, 12 weeks old)

Abbr. HO, homozygous; WT, wild type.

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Fig10. Lectin and alternative pathway activity of HO hMASP2 mice. 

LP activity in serum of age-matched female (A) and male (B) wild-type C57BL/6 and HO hMASP2 mice at different dilutions (n=3/group); (C) AP activity in serum of age-matched male wild-type C57BL/6 and HO hMASP2 mice at different dilutions (n=3/group).

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Fig11. LNP023 blocks alternative complement pathway activity in the serum of age-matched male wild-type C57BL/6 and HO hMASP2 mice (n=3/group).