Customized Genetically Engineered Mouse Models

Combined with versatile technical platforms, GenoBioTX guarantees every custom model created is intended to fulfill diversified requirements from researchers. We offer not only classic knockout and knockin models, but also conditional point mutations and conditional knockouts with reporters. Our custom models will be the cornerstone of your research

Comparison of Three Gene Modification Technologies

Technology

Key points

Duration

Applications

Pros and Cons

CRISPR/ Cas9

Cas9 nuclease cuts specific sites.

3-9 months

Gene knockout, conditional knockout, gene knock-in, point mutation, humanization.

Pros-Short project cycle, high efficiency. Lower risk of off-target effects through sequencing verification. Successive generations can remove off-target sites. Cons-Potential risk of off-target effects, but manageable by choosing appropriate sgRNA.

ESC Targeting

Homologous recombination modifies genes.

9-12 months

Gene knockout, conditional knockout, gene knock-in, point mutation, humanization.

Pros-Mature technology, stable results, no off-target effects. Can achieve large segment recombination Cons-Longer project duration.

PiggyBac Transposon Technology

Transposon integrates DNA segments into the genome.

3-6 months

Gene overexpression

Pros-Simple plamid insertion, high expression rate. Multiple insertion sites. Cons-Subsequent experiments require establishing stable lines.

Model Classifications

Gain of Function Loss of Function

Transgenic
• Random Transgenesis
• Targeted Transgenesis
Direct Overexpression
Conditional Overexpression
KI
• Knock - In
• Humanized Mice

Point Mutation
• Conventional Point Mutation
• Conditional Point Mutation
KO & CKO
• Conventional Knockout
• Conditional Knockout

Work Flow

What We Provide/Our Advantages

In-depth feasibility evaluation before your project starts

More rational model design to avoid artifactual phenotypes
More detailed project feasibility assessment
More accurate timelines

An efficient production line with rigorous quality control

Shortened project trunaround time
Guaranteed germline transmission of genetic modification in models generated from the ES cell targeting and CRISPR gene editing technologies

Regular and rigorous reporting during project implementation

A dedicated project manager
Seamless flow of information
One-on-one, face-to-face communication

A professional breeding support team and advanced animal breeding facilities

Animal health reports
Global delivery
Flexible delivery options

Technology	Duration	Pros & Cons
CRISPR/Cas9	3-9 mos	High efficiency, short cycle.
ESC Targeting	9-12 mos	No off-target effects, stable.
Transgenic	3-6 mos	Fast overexpression models.

Customized Genetically Engineered Mouse Models

Comparison of Three Gene Modification Technologies

Model Classifications

Work Flow

What We Provide/Our Advantages

In-depth feasibility evaluation before your project starts

An efficient production line with rigorous quality control

Regular and rigorous reporting during project implementation

A professional breeding support team and advanced animal breeding facilities

Technology Comparison

Work Flow

What We Provide / Our Advantages

In-depth feasibility evaluation

Efficient production line

Regular and rigorous reporting

Professional breeding support

Customized Genetically Engineered Mouse Models

Comparison of Three Gene Modification Technologies

Model Classifications

Work Flow

What We Provide/Our Advantages

In-depth feasibility evaluation before your project starts

An efficient production line with rigorous quality control

Regular and rigorous reporting during project implementation

A professional breeding support team and advanced animal breeding facilities

Technology Comparison

Work Flow

What We Provide / Our Advantages

In-depth feasibility evaluation

Efficient production line

Regular and rigorous reporting

Professional breeding support

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