Strain Model Specification

CT26-hCD24

Strain Name CT26-Cd24em1(hCD24)/Smoc
Catalog Number NM-S24A-TM03
Genetic Background CT26

Validation Data

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Figure 1. Expression of human CD24 on CT26-hCD24 cells was confirmed by flow cytometry.  

CT26-hCD24 cells and wild type CT26 cells were stained with species-specific anti- CD24 antibodies.  FACS analysis shows that human CD24 but not mouse CD24 was exclusively detectable on CT26-hCD24 cells.

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Figure 2. In vivo tumor growth curves in humanized CT26-hCD24 syngeneic model.

BALB/c mice were subcutaneously injected 1×106 CT26-hCD24 cells compared with wild type CT26 cells as control.  Tumor growth was monitored by measuring tumor size from day 5 after subcutaneous implantation.

Data shows that there were no significant differences between CT26-hCD24 cells and wild type CT26 cells in either tumorigenicity or tumor growth.

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Figure 3. FACS analysis of CD24 expression on tumor cells derived from humanized CT26-hCD24 syngeneic model with species-specific anti-CD24 antibodies.  

Data shows that human CD24 knock-in tumor exclusively express human CD24 but not mouse CD24.