hPDGFD

Fig.1 Detection of PDGFD expression in bladder by RT-PCR. 

Wild type: only one band at 198 bp with primers F1/R1(mPdgfd);

Homozygous: only one band at 235 bp with primers F2/R2(hPDGFD).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of PDGFD expression in hPDGFD knock-in mice by ELISA. 

The heart and kidney homogenates were collected from male 8-week-old wild-type C57BL/6 mice (WT, n=1) and homozygous hPDGFD knockin mice (HO, n=3), and analyzed by ELISA with PDGFD ELISA kit. Human PDGFD was detectable in homozygous hPDGFD mice. The ELISA kit is cross-reactive between human and mouse PDGFD protein.