hSIGLEC8(2)

Background : C57BL/6Smoc

Catalog : NM-HU-210033

Strain Name : C57BL/6Smoc-Siglecfem1(hSIGLEC8)Smoc

Gene Summary

Gene Name

Model Description

The endogenous mice Siglecf gene was replaced by human SIGLEC8 gene. While hSIGLEC8(Stock No.NM-HU-204983) mice function similarly to hSIGLEC8(2) mice,for more detailed information please contact our technical advisor.

Validation Data

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Fig1. Strain specific SIGLEC8 expression analysis in homozygouse hSIGLEC8 mice by flow cytometry. Spleen derived eosinophils were collected and analyzed by flow cytometry with species-specific anti-hSIGLEC8 and anti-mSIGLEC-F antibody. hSIGLEC8 was detectable in hSIGLEC8 mice but not in WT mice, mSIGLEC-F was detectable in WT mice but not in hSIGLEC8 mice.

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Fig2. Strain specific SIGLEC8 expression analysis in homozygouse hSIGLEC8 mice by flow cytometry. Eosinophils of PBMC were collected from WT and homozygouse hSIGLEC8 mice, and analyzed by flow cytometry with species-specific anti-hSIGLEC8 and anti-mSIGLEC-F antibody. hSIGLEC8 was detectable in a small proportion of eosinophils in hSIGLEC8 mice, and the mSIGLEC-F was not expressed.


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