1. In vitro Expression of H22-Luc-hGPC3 cell line
hGPC3 expression was measured in H22-Luc-hGPC3 cell by FACS . FACS results showed that significant hGPC3 expression on the H22-Luc-hGPC3 cell line.
Figure 1 hGPC3 expression in H22-Luc-hGPC3 cell line
2. Characterization of in vivo growth kinetics
To verify tumor formation of H22-Luc-hGPC3 cell line, cells were subcutaneously inoculated into female balb/c mice.
Figure 2. Tumor growth curve of H22-Luc-hGPC3 allogeneic model (n=5)
Figure 3. Body weight curve of H22-Luc-hGPC3 allogeneic model (n=5)
3. Expression of hGPC3 in tumors
To verify the hGPC3 protein expression, the tumors were dissected and separated for single cell suspension when he tumor volume is 500-1000mm3. Robust hGPC3 protein expression can be detected in the humanized modified tumors.
Figure 4. The expression of hGPC3 in humanized modified tumors by FACS
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