Knock-In Mouse Models

Knock-in mice are powerful tools used to introduce specific mutations or exogenous genes into a target locus. This allows for precise modeling of human genetic conditions and tracing of gene expression.

Knock-in Mouse Concept
  • Introduce reporter genes (GFP, RFP, Luciferase).
  • Introduce specific point mutations.
  • Construct humanized mouse models for drug testing.

Applications

Disease Modeling

Simulate human mutations to study the pathophysiology of rare diseases.

Drug Development

Test efficacy on humanized targets to reduce clinical trial risks.

Lineage Tracing

Identify cell fates and development stages using inducible reporters.

Knock-in Strategies

Replacement Type

Co-expression Type

Cre-LoxP Knock-in

Site-specific recombinase systems like Pvalb-2A-Cre allow for conditional control over gene expression.

Humanized Models

Direct insertion of human cDNA or full genomic replacement allows for the creation of mice that express human proteins for clinical validation.