Knock-in mice

Knock-in is the introduction of specific mutations or exogenous genes at the target gene locus.

Introduce a reporter gene at a specific site of the target gene.
Introduce point mutations into the target gene.
Construct humanized mouse models.

Knock-in mice applications

-Simulate human genetic mechanisms to explore disease mechanisms.
-Trace gene expression
-Conduct lineage tracing to trace cell origins.
-Humanize mouse genes to accelerate drug development.
-Introduce molecular genetic tools into mouse models.

Replacement Type

Co-expression Type

Pvalb-2A-Cre

-Cre recombinase is translated in cells expressing Pvalb and does not affect the endogenous expression of Pvalb.
-Can be used for studying neuronal differentiation.

Fluorescent protein/luciferase/protein tag

Fluorescent protein:Used for tracking gene expression.
Luciferase: Used for observing target gene expression in living organisms.
Protein tag: Suitable for situations where antibodies are not effective.

Humanized gene knock-in mouse

In mice, one or more genes are replaced by human genes, genomic sequences, or regulatory elements.

Replacement Type

Strategy of directly inserting human cDNA

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