Cd8a-2A-tdTomato-2A-Luc

Background : C57BL/6Smoc

Catalog : NM-KI-225025

Strain Name :

C57BL/6Smoc-Cd8aem(T2A-tdTomato-P2A-Luciferase)1Smoc

Gene Summary

Gene Name
  • Synonyms :

    Ly-2, Ly-B, Ly-35, Lyt-2, BB154331

  • NCBI ID :

    12525

  • MGI ID :

    88346

  • Ensembl ID :

    ENSMUSG00000053977

  • Strain State :

    Sperm cryopreservation

Model Description

T2A-tdTomato-P2A-Luciferase expression cassette was knocked into the Cd8a gene .

Validation Data

Fig.1.png

Fig.1 IVIS automatic exposure. According to the imaging process, the mice were injected with D-Luciferin, and the abdomen, back, and flanks were imaged in vivo. 

image.png

Fig.2 IVIS automatic exposure: ex vivo. According to the imaging process, D-Luciferin was injected, and the mouse brain, heart, lung, kidney, liver,  spleen, thymus, inguinal lymph node, axial lymph node (Axial LN), muscle, and the entire digestive system  (stomach to colon) were taken for imaging.

Fig.3.pngFig.3 Detection of tdTomato in mice thymus by FACS.

Fig.4.png

Fig.4 Detection of tdTomato in mice axillary lymph by FACS.

Fig.5.png

Fig.5 Detection of tdTomato in mice inguinal lymph by FACS.

Fig.6.pngFig.6 Detection of tdTomato in mice spleen by FACS.

Fig.7.pngFig.7 Detection of tdTomato in mice PB by FACS.

*The validation data above were completed in cooperation with Crownbio.

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