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Fig1. Analysis of ACE2 expression profile by qPCR in the ACE2 humanized mice. The homozygous and heterozygous KI mice express human ACE2 in lung, kidney and small intestine; while the heterozygous KI mice and wild type express mouse Ace2 on heart, lung, kidney and small intestine.
Fig2. Analysis of ACE2 gene expression in the lungs by RT-PCR in WT and ACE2 humanized mice. Mouse Ace2 mRNA was detectable by P7/8(446bp), human ACE2 mRNA was detectable by P5/6(283bp), M:100bp Plus DNA ladder from Transgen (BM311). The homozygous KI mouse expresses human ACE2 in the lungs, while the heterozygous KI mouse expresses both human ACE2 and mouse Ace2.
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