hCB1

Fig1. Detection of CB1 expression in cerebellum by RT-PCR. 

Wild type: only one band at 373 bp with primers F1/R1(mCb1);

Homozygous: only one band at 209 bp with primers F2/R2(hCB1);

Abbr. M, DNA marker; HO, homozygous;  WT, wild type.

Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig3. Detection of human CB1 expression in brain (Hippocampus) in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig4. Detection of human CB1 expression in CA1 in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig5. Detection of human CB1 expression in CA3 in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig6. Detection of human CB1 expression in dentate gyrus(DG) in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

Fig7. Detection of human CB1 expression in cerebellum in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.