Ctla8, Ctla-8, Il17, IL-17A
活体
Fig1. Analysis of IL17F gene expression in the kidney by RT-PCR. Mouse Il17f mRNA was detectable by P1/2(440bp), human IL17F mRNA was detectable by P3/4(259bp), M:100bp Plus DNA ladder from Transgen (BM311). The heterozygous KI mice express both human IL17F and mouse Il17f in the kidney.
Fig2. Analysis of IL17A gene expression in the spleen by RT-PCR in WT and IL17A/IL17F humanized mice . Mouse Il17a mRNA was detectable by P1/2(490bp), human IL17A mRNA was detectable by P3/4(443bp), M:100bp Plus DNA ladder from Transgen (BM311). The heterozygous KI mouse expresses both human IL17A and mouse Il17a in the spleen.
Fig. 3 Detection of hIL17A expression in serum, spleen and thymus homogenate by ELISA (n=3).
Abbr. HO, homozygous; WT, wild type.
Note. hIL17A/hIL17F and C57BL/6 mice were i.p. injected with LPS.
Fig. 4 Detection of hIL17F expression in serum, spleen and thymus homogenate by ELISA (n=3).
Abbr. HO, homozygous; WT, wild type.
Note. hIL17A/hIL17F and C57BL/6 mice were i.p. injected with LPS.
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