hSIGLEC10

Fig.1 Strain specific analysis of SIGLEC10 gene expression in WT and hSIGLEC10 mice by RT-PCR.

Mouse Siglecg mRNA was detectable in splenocytes of wild-type mice( P1/2 : 812bp). Human SIGLEC10 mRNA was detectable only in homozygous hSIGLEC10 mice (P3/2 : 394bp) , but not in the WT. 

M：100bp Plus DNA ladder from Transgen (BM311)

Fig.2 Strain specific SIGLEC10 expression analysis in the CD19+ PBMC or splenocytes of homozygous hSIGLEC10 mice by FACS. 

anti-mouse Siglec-G：BD Pharmingen™,563336；

anti-human Siglec-10：Biolegend，347603.

Fig.3 In vivo efficacy study in SIGLEC10 humanized mice grafted with MC38-hCD24 tumor model. CD24 mAb1 and CD24 mAb2 can inhibit the growth of MC38-hCD24 xenografts(A), and have no significant effect on the body weight of  hSIGLEC10 mice (B).