MC38-hBCMA

Background : MC38

Catalog : NM-S13-TM14

Strain Name :

MC38-Gt(ROSA)26Sorem1(hBCMA)/Smoc

Gene Summary

Gene Name

Model Description

These cells harbor a hBCMA cassette in the Rosa26 locus generated by homologous recombination.

Validation Data

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Figure 1. Schematic of the transgene insertion of a human BCMA cDNA cassette. Human BCMA gene is controlled by a CMA promoter.

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Figure 2. Expression of human BCMA on MC38-hBCMA cells was confirmed by flow cytometry.  

MC38-hBCMA cells and wild type MC38 cells were stained with species-specific anti- BCMA antibody.  FACS analysis shows that human BCMA expression on MC38-hBCMA cells was approximately 99%.

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Figure 3. In vivo tumor growth curves in humanized MC38-hBCMA syngeneic model.

C57BL/6 mice were subcutaneously injected MC38-hBCMA cells compared with wild type MC38 cells as control.  Tumor growth was monitored by measuring tumor size from day 6 after subcutaneous implantation.

Data shows that there were no significant differences between MC38-hBCMA cells and wild type MC38 cells in either tumorigenicity or tumor growth.

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Figure 4. FACS analysis of BCMA expression on tumor cells derived from humanized MC38-hBCMA syngeneic model with species-specific anti-BCMA antibodies.  

FACS analysis shows that human BCMA expression in human BCMA knock-in tumor  were approximately 68.14% (low dose) and 70.41% (high dose).


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