The model breeding that Shanghai Model Organisms can provide refers to the customized genetically modified animal models or the customer's existing genetically modified animal models. Through natural breeding or assisted reproductive breeding, specific genotype model products can be delivered within a specific period.
Specifically including: natural breeding , assisted reproductive breeding and SPF purification , etc.
Through model breeding, we can deliver a specified number of model strains with specific genotypes to customers at one time or in batches, effectively solving the technical or cost barriers that customers face in genotype identification, propagation cycle, animal quality, and operational management, and improving their research efficiency.
The superovulated female mouse ismated with the target male mouse, andtwo cells are taken for slow freezing onthe next day and stored in liquid nitrogen.
The survival rate of slow-frozen embryosafter thawing is high (more than 80%),and the pregnancy rate and litter rate oftransplanted mice are high.
The operation is difficult, and an expensive program freezer is required,and the operation time is up to 6 hours.
To thaw and transplant frozen embryosto obtain the required mice and rats.
The frozen embryos were taken out ofliquid nitrogen, thawed at roomtemperature,and put into thecorresponding resuscitation fluid forresuscitation.The surviving eggs weretransplanted into pseudo-pregnantfemale mice to obtain the required miceand rats.
Male mice are killed and sperm is takenout and stored in liquid nitrogen throughrapid freezing .
Fast operation speed and large amountof sperm.
In vitro fertilization is required duringrecovery,and the fertilization rate is low(generally 15%-25%).
To thaw frozen sperm to obtain therequired mice and rats.
The frozen sperm was taken out fromliquid nitrogen and placed at 37 degreesfor 10 minutes, then transferred to TYHfor sperm capacitation. The capacioussperm was used to obtain fertilized eggsthrough in vitro fertilization, which weretransplanted into pseudo-pregnantfemale mice to obtain the required mice add rats.
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