We provide model breeding and strain conservation services, as well as the customization of genetically engineered models through natural or assisted breeding.
The model breeding that GenoBioTX can provide refers to the customized genetically modified animal models or the customer's existing genetically modified animal models. Through natural breeding or assisted reproductive breeding, specific genotype model products can be delivered within a specific period.
Specifically including: natural breeding, assisted reproductive breeding and SPF purification, etc.
Through model breeding, we can deliver a specified number of model strains with specific genotypes to customers at one time or in batches, effectively solving the technical or cost barriers that customers face in genotype identification, propagation cycle, animal quality, and operational management, and improving their research efficiency.
Refers to a breeding method in which a specific genotype offspring model is obtained within a specific period of time through self-mating and breeding of animals under normal growth and development conditions and sexual cycles of the animal model.
It refers to a breeding method that uses assisted reproductive technologies such as egg superovulation, in vitro fertilization, and maternal surrogacy to obtain large quantities of offspring models of specific genotypes in the shortest possible time.
Refers to the process of obtaining SPF-level animal strains through embryo transfer.
Method
The super ovulated female mouse is mated with the target male mouse, and two cells are taken for slow freezing on the next day and stored in liquid nitrogen.
Advantages
The survival rate of slow-frozen embryos after thawing is high (more than 80%),and the pregnancy rate and litter rate of transplanted mice are high.
Disadvantages
The operation is difficult, and an expensive program freezer is required, and the operation time is up to 6 hours.
Objective
To thaw and transplant frozen embryos to obtain the required mice and rats.
Methods
The frozen embryos were taken out of liquid nitrogen, thawed at room temperature, and put into the corresponding resuscitation fluid for resuscitation.The surviving eggs were transplanted into pseudo-pregnant female mice to obtain the required mice and rats.
Method
Male mice are killed and sperm is taken out and stored in liquid nitrogen through rapid freezing.
Advantages
Fast operation speed and large amount of sperm.
Disadvantages
In vitro fertilization is required during recovery, and the fertilization rate is low (generally 15%-25%).
Objective
To thaw frozen sperm to obtain the required mice and rats.
Methods
The frozen sperm was taken out from liquid nitrogen and placed at 37 degrees for 10 minutes, then transferred to TYH for sperm capacitation. The capacious sperm was used to obtain fertilized eggs through in vitro fertilization, which were transplanted into pseudo-pregnant female mice to obtain the required mice add rats.
Unlock the full potential of your research with our therapeutic targeted animal models. Contact us today to discuss your project and discover how we can help you achieve your scientific goals. Together, let's push the boundaries of biomedical research and make a lasting impact on human health.
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